C (3-6 months) C（3〜6か月） <a href='https://brc.riken.jp/mus/pcr00457'>Genotyping protocol -PCR-</a> 中山　敬一 Keiichi NAKAYAMA ＞10世代　C57BL/6に戻し交配 Developed by Dr. Keiichi Nakayama, Kyusyu University. Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://www.bioreg.kyushu-u.ac.jp/saibou/index_en.html" target="_blank">Lab HP</A> true PKCδ Knockout mice PKCδ Knockout mice PGK promoter (mouse phosphoglycerate kinase gene promoter), Neomycin resistance gene (neomycin phosphotransferase II (nptll) gene of the transposon Tn5 in Escherichia coli K12), PGK polyA (mouse phosphoglycerate kinase gene terminater), mouse Prkcd genomic DNA B6.129P2-Prkcd<tm1Kin> B6.129P2-Prkcd<tm1Kin> Backcross to C57BL/6 (Heterozygote x C57BL/6JJcl) Kyushu Univ. Protein kinase C (PKC) delta is a member of PKC family which phosphorylates a wide variety of protein targets and is known to be involved in diverse cellular signaling pathways. In PKC family members, PKC delta has a unique function to supress cell proliferation. PKC-delta deficient mice exhibit expansion of the B- lymphocyte population and show autoimmune disease after 6 months of age. 国立大学法人九州大学 The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>共同研究 RBRC00457